Centriole assembly is the process by which centrioles, which are small, barrel-shaped organelles located in the cytoplasm of animal cells, are formed. Centrioles play a crucial role in cell division by organizing the spindle apparatus, which helps to separate chromosomes during cell division. During centriole assembly, new centrioles are formed through a highly regulated process involving the duplication of existing centrioles. This process typically begins in the S phase of the cell cycle, when the centrioles duplicate to form a pair of centrosomes. These centrosomes then migrate to opposite ends of the cell and help to organize the mitotic spindle during cell division. Centriole assembly is a tightly controlled process involving the recruitment of specific proteins and regulatory factors to the centriolar region. Dysregulation of centriole assembly can lead to errors in cell division and contribute to the development of diseases such as cancer. Research in the field of centriole assembly aims to understand the molecular mechanisms underlying centriole formation and how these processes are regulated in normal and diseased cells. This knowledge can help to uncover new therapeutic targets for the treatment of diseases involving centriole dysfunction.